DNA Vectors
System Biosciences LLC SYSTEM BIOSCIENCES LLC Reporter Plasmid, TCF/LEF, rFluc, GFP, 10 μg
The pGreenFire 2.0 TCF/LEF Reporter is designed for studying TCF/LEF signaling using a red firefly luciferase (rFluc) and GFP reporter system, enabling reliable stable cell line generation. It allows for sorting responsive cells with GFP and measuring activity with red firefly luciferase.
- Designed to study TCF/LEF signaling pathways.
- Utilizes a red firefly luciferase (rFluc) and GFP reporter system.
- Engineered for reliable stable cell line generation, including difficult-to-transfect cells.
- Allows sorting of responsive cells with GFP and quantitative measurement with rFluc.
- Monitors signal transduction in real time with an upgraded design.
- Features TCF/LEF transcriptional response elements and a minimal CMV promoter.
- Includes a constitutive selection cassette with a puromycin resistance gene.
BIOHIPPO AAV-hSyn-EGFP-5'miR-30-shRNA(scramble)-3'-miR30-WPREs
Recombinant adeno associated virus expressing 5'miR-30-shRNA(scramble)-3'miR-30 fused to EGFP under the hSyn promoter with WPRE and hGH polyA elements. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
Expression Systems PVL1393 TRANSFER VECTOR
pVL1392 and pVL1393 are transfer vectors designed for high-level expression of a cloned gene driven by the strong Autographa californica multiple nucleopolyhedrovirus (AcMNPV) polyhedrin promoter (pPolh).
pVL1392 and pVL1393 are pUC8 based plasmids containing the AcMNPV polyhedrin locus lacking the majority of the polyhedrin open reading frame. A multiple cloning site (MCS) sequence has been inserted 37 nucleotides downstream from the original polyhedrin ATG start codon, which has been mutated to ATT.
pVL1392 and pVL1393 share identical sequences, with the exception that they each have the same MCS in opposing orientations. A start and stop codon must be provided with the gene that is to be cloned into the MCS. For maximal recombinant protein expression, the distance between the cloning site and the ATG start of the inserted gene should be kept to a minimum, and should not exceed 100 nucleotides.
Vector Biolabs AAV-CAG-GFP (AAV-DJ) AAV (AAV/DJ-CAG-GFP)
Ready to use in vivo grade AAV and Adenovirus Ready to use in vivo grade AAV and Adenovirus pre synthesized DNA vector High titer viral vectors 1/EA
Vector Biolabs GFP expressing RGD fiber modified Adenovirus (Ad(RGD)-CMV-GFP)
Ready to use in vivo grade AAV and Adenovirus Ready to use in vivo grade AAV and Adenovirus pre synthesized DNA vector High titer viral vectors 1/EA
Sino Biological pCMV3-untagged Negative Control Vector
Negative control for the pCMV3-untagged clone.
BIOHIPPO AAV-GAD67-eNpHR3.0-EGFP-WPREs
Recombinant adeno associated virus expressing eNpHR3.0 fused to EGFP under the GAD67 promoter with WPRE and hGH polyA elements. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
BIOHIPPO AAV-tie2-Cre-P2A-mCherry-WPREs
Recombinant adeno associated virus expressing Cre fused to mCherry under the Tie2 promoter with WPRE and hGH polyA elements. The Cre dependent configuration enables expression only after recombination, providing precise targeted control of targeted neurons. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
